Engineering Tutorials

BME – 201 F BIOCHEMISTRY

SECTION A

Unit-1. Introduction to Biochemistry: Cell, eukaryotic cell structure, functional role of each organelle, sub cellular fractionation: Differential centrifugation, transport of substances across biological membrane functions.

SECTION B

Unit-2. Enzymes (Proteins): Chemical nature of enzymes (Proteins), general properties of enzymes, diagnostic enzymes, spectrophotometric measurement of enzymes (proteins) isolation method study, enzyme biotechnology.

Nucleic Acids: Composition and functions of nucleic acids (A brief account) genes, outlines of DNA structure, recombinant DNA and its applications.

SECTION C

Unit-3. Instrumentation: Principles and applications of photometry, spectrophotometry, fluorometry, nephalometry and turbidimetry, Spectroscopy: Basic concepts and instrumentation, IR (IR FTIR), NMR (including FTNMR), and UV. Biochemical analysis carried out in the estimation of blood constituents like glucose, urea, creatinine, protein, cholesterol, bilirubin etc. and normal and abnormal contents of urine, Separation of serum proteins by electrophoresis, Automation in biochemical analysis.

SECTION D

Unit 4. Acid base Homeostasis: Acids, bases, measurement of pH and glass electrodes, role of kidney and lungs in acid base balance, biochemical measurement of acid base balance, disorders of acid-base balances using blood gas analyzer. Isotopes: Definitions, units, radioactive isotopes, applications of isotopes in life sciences and medicine.

Texts:

1. Organic Spectroscopy-Principles and applications by Jagmohan, Narosa Publish House

2. A textbook of biochemistry-A.V.S.S. Rama Rao (UBSPD)

3. Instant notes on Biochemistry-Hooper et.al.

4. Enzymes-Biochemistry, Biotechnology, Clinical chemistry-Trenor Palmer(EWP)

Reference :

1. Organic Analytical chemistry-Theory and practice by Jagmohan. Narosa publishing

2. Harper’s Biochemistry, 25^th edition (McGraw Hill)

3. Fundamentals of biochemistry-J.L. Jain, Sanjay Jain (S.Chand)

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